Western Blot: PGRMC2 Antibody [NBP1-49895] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PGRMC2 antibody NBP1-49895 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PGRMC2 was also immunoprecipitated by rabbit anti-PGRMC2 antibody NBP1-49716, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).